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Peptide stability in solution: Once dissolved in water, peptide bonds can slowly hydrolyze

Cells were lysed as previously described (Mukanganyama et al., Preparation of Alkylated GSTs Using iodo-APAP or IAM Reductive alkylation of human GSTA1, M1, M2, or P1 (1.83.6 nmol) was performed in 100 mM ammonium bicarbonate (ABC, pH 8.5) using DTT (250 nmol

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